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KMID : 0350519930460041715
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1715 ~ p.1727
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Detection of Human Papillomavirus and DNA Ploidy Analysis for the Prognosis of Laryngeal Cancer
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Abstract
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In the treatment of laryngeal cancer, the most important factor that affects the prognosis is the local and cervical recurrence of tumor. Fit is still unclear why comparably staged patients with squamous cell carcinoma of the larynx have such
diverse
clinical courses and responses to similar treatment. Although the clinical outcome is greatly influenced by the stage of disease, the current staging system is an imperfect prognostic indicator.
In this study we investigated the relationships between the presence of human papillomavirus (HPV) and DNA ploidy to the prognosis of laryngeal cancer and the possibility of using these parameters for prognosis. The subjects of this study were 42
patients with laryngeal cancer who visited the Department of Otolaryngology, Kangnam St. Mary´s Hospital, Catholic University Medical College from March, 1988 to Feburay, 1992, for whom laryngectomy was performd.
To investigate the presence of HPV DAN in the cancer tissue, polymerase chain reaction(PCR) amplification was used with the conserved primer that can amplify the E1 open reading frame of HPV genome. DNA ploidy was determined by flow
cytometry(FCM).
We obtained the following results from analysis of the results and of the factors such as detection of HPV DNA, DNA analysis obtained from FCM, clinical features and histologic findings.
1. HPV DNA was detected in 12 cases among the 42 patients(28.5%) and the detection rate was higher in the tumors that infiltrate less than 1 cm to the parenchyme.
2. Most of the aneuploid tumor was the advanced stage and showed enhanced infiltrative tendency to adjacent tissue and the cervical metastasis.
3. There was no correlation between the ploidy and the presence of HPV DNA.
4. The recurrence of cancer was higher either in the presence of HPV DNA or in aneuploid tumor.
5. The recurrence of the aneuploid tumor combined with the presence of HPV DNA occurred more frequently in the cervical region than in the primary site.
From these results, we concluded that the HPV infection does not affect the ploidy of tumor in laryngeal cancer, and the presence of HPV DNA and aneuploidy of the tumor might be related with the recurrence of laryngeal cancer.
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KEYWORD
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